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A Novel Enzymatic Method for Measuring Mizoribine 5′-Monophosphate Levels in Serum

Paper ID Volume ID Publish Year Pages File Format Full-Text
21531 43226 2008 4 PDF Available
Title
A Novel Enzymatic Method for Measuring Mizoribine 5′-Monophosphate Levels in Serum
Abstract

Inosine 5′-monophosphate dehydrogenases (IMPDH) from Oceanobacillus iheyensis and Bacillus subtilis were biochemically characterized with the aim of establishing a mizoribine 5′-monophosphate (MZR-P) assay. MZR-P is the active metabolite of mizoribine, which has been successfully used as an immunosuppressive agent. A sensitive method for measuring the MZR-P concentration in serum, based on its ability to inhibit IMPDH, was developed. The method was applied to an automatic clinical analyzer, enabling measurement of MZR-P levels in a set of serum samples. MZR-P at concentrations of 0.1–13.6 (μg/ml (0.3–40 μM) in the samples could be measured with 0.7% within-run coefficients of variation.

Keywords
inosine 5′-monophosphate dehydrogenase; mizoribine; mizoribine 5′-monophosphate; Oceanobacillus iheyensis; Bacillus subtilisIMP, inosine 5′-monophosphate; MZR, mizoribine; MZR-P, mizoribine 5′-monophosphate
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A Novel Enzymatic Method for Measuring Mizoribine 5′-Monophosphate Levels in Serum
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Bioscience and Bioengineering - Volume 106, Issue 5, November 2008, Pages 511–514
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us