fulltext.study @t Gmail

Onset timing of transient gene expression depends on cell division

Paper ID Volume ID Publish Year Pages File Format Full-Text
21543 43227 2010 5 PDF Available
Title
Onset timing of transient gene expression depends on cell division
Abstract

By using the time lapse of both phase-contrast and fluorescent images, we examined the morphology of cells and the dynamics of gene expression (EGFP). We applied k-means clustering to the time course data of fluorescent intensity of EGFP and successfully found four subpopulations.Discriminating the appropriate clusters and investigating the details of them, we found that almost all cells express the transfected gene after cell division and also found there is a strong correlation between onset timing and cell division. This result suggested that it is possible to normalize the dynamics of gene by arranging the onset timing of gene expression or by arranging the cell division.

Keywords
Cellular dynamics; Onset timing; Single cell analysis; Time course; Transfection mechanism; Synchronization; Gene expression
First Page Preview
Onset timing of transient gene expression depends on cell division
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us
Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Bioscience and Bioengineering - Volume 109, Issue 1, January 2010, Pages 62–66
Authors
, , ,
Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us