fulltext.study @t Gmail

Refined method for the genomic integration of complex synthetic circuits

Paper ID Volume ID Publish Year Pages File Format Full-Text
21554 43228 2010 8 PDF Available
Title
Refined method for the genomic integration of complex synthetic circuits
Abstract

Genetic reconstruction of regulatory gene circuits is currently applied in systematic dynamics and structure–function studies of intact cellular networks in systems biology. We present a modified procedure for the integration of complex genetic circuits into the Escherichia coli genome, to provide an efficient synthetic approach for stochastic study and the artificial engineering of genetic networks. Linear artificial sequences of various lengths were easily integrated into the bacterial genome at one time. Comparison of the cellular concentrations of proteins encoded by genes carried on plasmids or the genome indicated that genome recombination could minimize the copy number noise in the genetic circuit, allowing precise design and interpretation of the cellular network. The refined recombination procedure allowed efficient construction of a single copy of a complex genetic circuit in cells, and the resultant reduced fluctuation in copy number led to accurate phenotypic behaviour of the genome-integrated synthetic switch corresponding to the design principle. The availability of long-fragment insertions makes the reconstruction of complex networks easy on the genome, and provides a powerful tool for precise engineering in synthetic and systems biology.

Keywords
Doxy, doxycycline; GFP, green fluorescent protein; IPTG, Isopropyl β-d-1-thiogalactopyranoside; PCR, polymerase chain reaction; RFP, red fluorescent proteinSynthetic approach; Genetic engineering; Genome recombination; Systems biology; Noise; Hysteresis;
First Page Preview
Refined method for the genomic integration of complex synthetic circuits
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us
Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Bioscience and Bioengineering - Volume 110, Issue 5, November 2010, Pages 529–536
Authors
, , , ,
Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us