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Taxol promising fungal endophyte, Pestalotiopsis species isolated from Taxus cuspidata

Paper ID Volume ID Publish Year Pages File Format Full-Text
21556 43228 2010 6 PDF Available
Title
Taxol promising fungal endophyte, Pestalotiopsis species isolated from Taxus cuspidata
Abstract

The endophytic fungi, Pestalotiopsis versicolor and Pestalotiopsis neglecta, were isolated from the healthy leaves and bark of the Japanese Yew tree, Taxus cuspidata. The fungal species were identified by their characteristic culture morphology and molecular analysis. For the first time, the test fungi were screened for the production of taxol in modified liquid medium. The presence of taxol was confirmed by HPLC, 1H NMR, and LC–MS methods of analysis. The maximum amount of taxol production in P. versicolor was recorded as 478 μg/l. The production rate was increased to 9560-fold than that found in the culture broth of earlier reported fungus, Taxomyces andreanae. The extracted fungal taxol showed a strong cytotoxic activity in the in vitro culture of tested human cancer cells by apoptotic assay indicating that the increase in taxol concentration induces increased cell death. A PCR-based screening for ts, a unique gene in the formation of the taxane skeleton, confirmed the molecular blueprint for taxol biosynthesis. The results designate that the fungal endophyte, P. versicolor, is an excellent candidate for an alternate source of taxol supply and can also serve as a potential species for genetic engineering to enhance the production of taxol to a higher level.

Keywords
HPLC, high-performance liquid chromatography; PCR, polymerase chain reaction; ts, taxadiene synthase; PDA, potato dextrose agar; BSL, Bio-System Laboratory; UV, ultraviolet; LC–MS, liquid chromatography–mass spectroscopy; 1H NMR, proton nuclear magnetic r
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Taxol promising fungal endophyte, Pestalotiopsis species isolated from Taxus cuspidata
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Bioscience and Bioengineering - Volume 110, Issue 5, November 2010, Pages 541–546
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us