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Regulation and improvement of triterpene formation in plant cultured cells of Eriobotrya japonica Lindl

Paper ID Volume ID Publish Year Pages File Format Full-Text
21564 43228 2010 5 PDF Available
Title
Regulation and improvement of triterpene formation in plant cultured cells of Eriobotrya japonica Lindl
Abstract

ABSTRACTLoquat (Eriobotrya japonica Lindl) is a traditional Chinese medicinal plant that contains triterpenes, which have been shown to exhibit pharmaceutical activities. In this study, we investigated various different culture conditions for cultured cells of loquat to produce triterpenes, including illumination, carbon source, nutrient composition and culture system. When cultured on 2.5 mg/l of 6-benzyladenine, 1 mg/l of naphthalene acetic acid and 30 g/l of sucrose at 25 ± 2 °C in the dark for 30 days, the nutrient composition significantly regulated the cell growth and triterpene production. Supplied with the Murashige and Skoog medium reached higher level of dry weight (1.27 ± 0.09 g per flask) and total triterpene production (151.54 ± 12.58 mg/g of cultured cells), and the N6 medium produced tormentic acid but inhibited other triterpene products, while the B5 medium produced relatively high corosolic acid. Also found, suspension cultures of loquat cell could achieve high productivity as callus culture.

Keywords
BA, 6-Benzyladenine; NAA, Naphthalene acetic acid; HPLC, High performance liquid chromatography; TA, Tormentic acid; MA, Maslinic acid; CA, Corosolic acid; OA, Oleanolic acid; UA, Ursolic acid.Loquat; Triterpenes; Tissue culture; Secondary metabolite; Sus
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Bioscience and Bioengineering - Volume 110, Issue 5, November 2010, Pages 588–592
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us