fulltext.study @t Gmail

Spatial control of cells, peptide delivery and dynamic monitoring of cellular physiology with chitosan-assisted dual color quantum dot FRET peptides

Paper ID Volume ID Publish Year Pages File Format Full-Text
2157 101 2010 9 PDF Available
Title
Spatial control of cells, peptide delivery and dynamic monitoring of cellular physiology with chitosan-assisted dual color quantum dot FRET peptides
Abstract

Cell-based assays have become important tools in the pharmaceutical and biotechnology industries. However, observing and monitoring molecules in cells that mimic the physiological environment is often difficult. Dynamic processes not only increase the accuracy of simulations, but also improve our understanding of the function and regulation of molecules within cells. In this study we used chitosan as a multifunctional biomaterial for selective micropatterning of cells, peptide delivery and covalent bonding with quantum dots (QD) to decrease the cytotoxicity of QD. Our results demonstrate the efficacy of chitosan–QD–peptide–Alexa Fluor 488 in controlling the spread and spatial organization of cells. Cationic chitosan also provided an efficient delivery mechanism to live cells. We used the shift from green to red fluorescence of the chitosan dual color QD peptide to detect biological activity. This methodology has potential applications in high throughput screening of inhibitors and activators of biological mechanisms and pathways and for use in the pharmaceutical industry.

Keywords
Chitosan; Micropatterning; Peptide delivery; Quantum dots; FRET
First Page Preview
Spatial control of cells, peptide delivery and dynamic monitoring of cellular physiology with chitosan-assisted dual color quantum dot FRET peptides
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us
Publisher
Database: Elsevier - ScienceDirect
Journal: Acta Biomaterialia - Volume 6, Issue 9, September 2010, Pages 3621–3629
Authors
, , , , ,
Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us