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Physiological role and redox properties of a small cytochrome c5, cytochrome c-552, from alkaliphile, Pseudomonas alcaliphila AL15-21T

Paper ID Volume ID Publish Year Pages File Format Full-Text
21589 43230 2009 6 PDF Available
Title
Physiological role and redox properties of a small cytochrome c5, cytochrome c-552, from alkaliphile, Pseudomonas alcaliphila AL15-21T
Abstract

Cytochrome c-552 from Pseudomonas alcaliphila AL15-21T, which is a small cytochrome c5 from Pseudomonas spp., was first purified and characterized in our previous study. Although it has been found that cytochrome c-552 is induced at a high pH under air-limited condition, the physiological role of this cytochrome c has not been clarified yet. Therefore, to understand its physiological role, further characterization of this cytochrome expressed in Escherichia coli was performed. The yield of the recombinant protein reached 2.8 mg/l of culture, which was 76.4-fold larger than that of native cells. Analytical data of the recombinant protein exactly agreed with that of native cytochrome c-552. The recombinant cytochrome c-552 was oxidized by partially purified cb-type cytochrome c oxidase from P. alcaliphila AL15-21T at a rate of 9.6 μ mol min− 1 mg oxidase− 1. Unlike reported cytochromes c from other Pseudomonas spp., the E°' values between pHs 5.0 and 10.0 were nearly unchanged. Cytochrome c-552 oxidized very slowly at pHs 8.0 (6.1 × 10− 4 h− 1), 9.0 (1.4 × 10− 3 h− 1) and 10.0 (1.6 × 10− 3 h− 1), whereas it oxidized more rapidly at pH 7.0 (2.5 × 10− 3 h− 1). On the other hand, horse heart cytochrome c showed higher oxidation rates at pHs 6.0–10.0 than cytochrome c-552. It is considered that the high electron-retaining ability of cytochrome c-552 at high pHs is important for its physiological function in the environmental adaptation of this bacteria for superior growth at high pHs under air-limited conditions.

Keywords
Cytochrome c5; Expression; Alkaliphilic; Pseudomonas alcaliphila; cb-type cytochrome c oxidase
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Physiological role and redox properties of a small cytochrome c5, cytochrome c-552, from alkaliphile, Pseudomonas alcaliphila AL15-21T
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Bioscience and Bioengineering - Volume 108, Issue 6, December 2009, Pages 465–470
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us