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Oxidative/heat stress enhanced production of chitosanase from Streptomyces griseus cells through its interaction with liposome

Paper ID Volume ID Publish Year Pages File Format Full-Text
21590 43230 2009 6 PDF Available
Title
Oxidative/heat stress enhanced production of chitosanase from Streptomyces griseus cells through its interaction with liposome
Abstract

The effective production and secretion of chitosanase from S. griseus cells, in the presence of hydrogen peroxide, were studied by the treatment of the cells with 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphatidylcholine (POPC) liposomes under heat condition. The variation of the conformation and activity of the chitosanase caused by the interaction of liposomes with target chitosanase under stress condition was systematically investigated by using circular dichroism (CD) spectra and dielectric dispersion analysis (DDA). The effect of the oxidative stress (hydrogen peroxide) on the lipid and protein peroxide of cell membrane of S. griseus pretreated with and without liposomes under heat stress at 41 °C was further carried out. The possible utilization of membrane–membrane interaction between liposomes and cell membrane induced by the heat treatment was further investigated to enhance the production of chitosanase from S. griseus under oxidative stress condition.

Keywords
Membrane stress biotechnology; Chitosanase; Liposome; Oxidative stress; Streptomyces griseus
First Page Preview
Oxidative/heat stress enhanced production of chitosanase from Streptomyces griseus cells through its interaction with liposome
Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Bioscience and Bioengineering - Volume 108, Issue 6, December 2009, Pages 471–476
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering