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Preparation of coculture system with three extracellular matrices using capillary force lithography and layer-by-layer deposition

Paper ID Volume ID Publish Year Pages File Format Full-Text
21603 43230 2009 7 PDF Available
Title
Preparation of coculture system with three extracellular matrices using capillary force lithography and layer-by-layer deposition
Abstract

Micropatterned cocultures were fabricated with 3 extracellular matrices, hyaluronic acid (HA), fibronectin, and collagen. The feature of the fabrication processes is to avoid the use of potentially cytotoxic materials and utilize capillary force of the solution and interactions between the extracellular matrix components. The coculture system can be used to investigate the effects of heterocellular interactions on cellular fate. Direct heterocellular connections between hepatocytes and fibroblasts were visualized by the transcellular diffusion of fluorescein in this coculture system. The interactions between hepatocytes and fibroblasts were crucial for the maintenance of albumin synthesis by hepatocytes. The coculture system was also beneficial for investigating the effects of cell–cell interactions on the induction of embryonic stem (ES) cell differentiation. In cocultures grown in a sea-island pattern, ES cells formed isolated colonies surrounded by PA6 cells and differentiated into neurons with branched neurites that extended from the colonies. This versatile and biocompatible coculture system could potentially be a powerful tool for investigating cell–cell interaction and for tissue engineering applications.

Keywords
Micropatterned coculture; Hepatocyte; Fibroblast; Neuron; Hyaluronic acid
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Preparation of coculture system with three extracellular matrices using capillary force lithography and layer-by-layer deposition
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Bioscience and Bioengineering - Volume 108, Issue 6, December 2009, Pages 544–550
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us