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Molecular characterization of the PEX14 gene from the methylotrophic yeast Pichia methanolica

Paper ID Volume ID Publish Year Pages File Format Full-Text
21607 43231 2011 4 PDF Available
Title
Molecular characterization of the PEX14 gene from the methylotrophic yeast Pichia methanolica
Abstract

In this study, we describe the molecular characterization of the PmPEX14 gene encoding the peroxisomal membrane protein from the methylotrophic yeast Pichia methanolica. The pex14Δ strain of P. methanolica lost its ability to grow on methanol and oleate but grew normally on glucose. Disruption of the PmPEX14 caused a decrease in the activities of peroxisomal methanol-metabolizing enzymes and mislocalization of those proteins into the cytosol and vacuole. Taken together, these findings show that PmPex14p has an essential physiological role in methanol metabolism in P. methanolica.

Keywords
Pichia methanolica; Pex14p; Peroxisome; Alcohol oxidase isozymes
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Molecular characterization of the PEX14 gene from the methylotrophic yeast Pichia methanolica
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Bioscience and Bioengineering - Volume 111, Issue 6, June 2011, Pages 624–627
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
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Any Questions? feel free to contact us