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Enhanced plumbagin production in elicited Plumbago indica hairy root cultures

Paper ID Volume ID Publish Year Pages File Format Full-Text
21622 43231 2011 5 PDF Available
Title
Enhanced plumbagin production in elicited Plumbago indica hairy root cultures
Abstract

Elicitation of Plumbago indica hairy roots with yeast carbohydrate fraction, chitosan, manganese chloride, copper chloride and methyl jasmonate exhibited significant elevation (~ 1.2 to 2 fold) of plumbagin production in shake flask culture as compared with control. Chitosan and methyl jasmonate elicitation also caused simultaneous plumbagin leaching into culture media. Three days’ exposure of chitosan (200 mg l−1) and methyl jasmonate (80 μM) together synergized total plumbagin yield to its maximum 11.96 ± 0.76 mg g−l DW in shake flask culture. In bioreactor cultivation, a significant raise in fresh root biomass was recorded on day 20 as compared with control shake flask culture. Three days’ exposure of chitosan (200 mg l−1) and methyl jasmonate (80 μM) with 20 days old bioreactor-culture significantly improved total plumbagin production to 13.16 ± 1.72 mg g−l DW with simultaneous plumbagin leaching into bioreactor media.

Keywords
Plumbago indica; Hairy roots; Elicitors; Bioreactor; Chitosan; Methyl jasmonate
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Enhanced plumbagin production in elicited Plumbago indica hairy root cultures
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Bioscience and Bioengineering - Volume 111, Issue 6, June 2011, Pages 706–710
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us