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Purification of polygalacturonase from solid-state cultures of Aspergillus carbonarius

Paper ID Volume ID Publish Year Pages File Format Full-Text
21627 43232 2010 6 PDF Available
Title
Purification of polygalacturonase from solid-state cultures of Aspergillus carbonarius
Abstract

ABSTRACTPurification of polygalacturonase (PG) from the cultures of Aspergillus carbonarius obtained by acetate buffer extraction after solid-state fermentation was attempted by integrated membrane process and alginate affinity precipitation. The carbohydrates were completely eliminated (98%–99%) with a PG recovery of 72%–80% during integrated membrane process, which would otherwise interfere with the purification process and lead to enzyme loss. However, specific activity of PG did not improve (1.19–1.21 fold) due to the presence of other similar molecular mass proteins. Under optimum conditions of affinity precipitation, the specific activity of PG enhanced to 2450 U/mg (4 fold) with almost complete elimination of carbohydrates and colour compounds resulting in a PG recovery of 61%. PG purity obtained with ultrafiltration (UF) was comparable with the conventional dialysis during desalting eluted PG, besides UF rendered a concentrated PG. The enzyme purity stated was as descend by SDS–PAGE. The results suggested suitability of affinity precipitation for PG purification from solid-state cultures and the potential of UF as a single step process for handling eluted PG.

Keywords
Aspergillus carbonarius; Polygalacturonase; Solid-state fermentation; Integrated membrane process; Alginate affinity precipitation; Ultrafiltration; Desalting
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Purification of polygalacturonase from solid-state cultures of Aspergillus carbonarius
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Bioscience and Bioengineering - Volume 109, Issue 2, February 2010, Pages 101–106
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us