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Characterization of microorganisms at different landfill depths using carbon-utilization patterns and 16S rRNA gene based T-RFLP

Paper ID Volume ID Publish Year Pages File Format Full-Text
21632 43232 2010 8 PDF Available
Title
Characterization of microorganisms at different landfill depths using carbon-utilization patterns and 16S rRNA gene based T-RFLP
Abstract

A borehole core from 20 m depth of a Japanese landfill was characterized chemically and microbially. The borehole core sample was typically divided into 5 waste layers; 2.4–4.0 m, 5.7–8.5 m, 9.25–9.6 m, 9.77–14.9 m, and 15.9–17.86 m depths. The waste layers' ages spanned about 14 years between the bottom and top. Archaeal 16S rRNA gene and eubacterial 16S rRNA gene in the waste samples at their respective levels were 9.8 × 105–7.2 × 107 and 1.2 × 107–7.2 × 109 copy/g-wet. Similar to populations of viable and culturable bacteria, those populations were high at 7.0 m and 17.5 m depth, but low at 3.0 m depth. The microorganisms' phenotypes and genotypes were evaluated, respectively, using carbon-utilization tests and by eubacterial 16S rRNA gene based T-RFLP. Low dominance of the VFA-utilizing bacteria in samples and low concentrations of VFAs in all waste layers suggest that the organic decomposition in this landfill site remained. Gamma-proteobacteria dominated the microbial community at 17.5 m depth. Clostridia were detected at 7.0, 11.5, and 17.5 m depths, suggesting strict anaerobic conditions in these deep layers. The Shannon–Weaver diversity index showed lower values at 3.0 m and 11.5 m depth with a T-RF pattern. The diversity index calculated from the carbon-utilization pattern increased slightly with depth at the landfill site. The landfill-site waste layers are expected to be mutually isolated and to form unique microbial communities depending on the buried wastes' composition, temperature, moisture content, and pressure inside the landfill.

Keywords
Landfill; Borehole core of waste; T-RFLP; Microbial community; Carbon-utilization patterns; Microbial populations
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Characterization of microorganisms at different landfill depths using carbon-utilization patterns and 16S rRNA gene based T-RFLP
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Bioscience and Bioengineering - Volume 109, Issue 2, February 2010, Pages 130–137
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us