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The impact of the overexpression of human UDP-galactose transporter gene hUGT1 in tobacco plants

Paper ID Volume ID Publish Year Pages File Format Full-Text
21637 43232 2010 11 PDF Available
Title
The impact of the overexpression of human UDP-galactose transporter gene hUGT1 in tobacco plants
Abstract

When the human UDP-galactose transporter 1 gene (hUGT1) was introduced into tobacco plants, the plants displayed enhanced growth during cultivation, and axillary shoots had an altered determinate growth habit, elongating beyond the primary shoots and having a sympodial growth pattern similar to that observed in tomatoes at a late cultivation stage. The architecture and properties of tissues in hUGT1-transgenic plants were also altered. The leaves had an increase in thickness, due to an increased amount of spongy tissue, and a higher content of chlorophyll a and b; the stems had an increased number of xylem vessels and accumulated lignin and arabinogalactan proteins (AGPs). Some of these characteristics resembled a gibberellin (GA)-responsive phenotype, suggesting involvement of GA. RT-PCR-based analysis of genes involved in GA biosynthesis suggested that the GA biosynthetic pathway was not activated. However, an increase in the proportion of galactose in polysaccharide side chains of AGPs was detected. These results suggested that because of higher UDP-galactose transport from the cytosol to the Golgi apparatus, galactose incorporation into polysaccharide side chains of AGP is involved in the gibberellin response, resulting in morphological and architectural changes.

Keywords
Nucleotide-sugar transporter; Gibberellin; Arabinogalactan protein; Golgi apparatus; Sugar chain
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The impact of the overexpression of human UDP-galactose transporter gene hUGT1 in tobacco plants
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Bioscience and Bioengineering - Volume 109, Issue 2, February 2010, Pages 159–169
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
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