fulltext.study @t Gmail

Quantification and improvement of error rate during ligase detection reaction

Paper ID Volume ID Publish Year Pages File Format Full-Text
21645 43232 2010 3 PDF Available
Title
Quantification and improvement of error rate during ligase detection reaction
Abstract

We estimated the actual error rate during ligase detection reaction (LDR), and confirmed that DNA sequences around 3′ ends are greatly influenced on the specificity of LDR. Its specificity is increased about 1000 times by introducing a mismatch base near the 3′ ends.

Keywords
LDR; SNP; Specificity; Thermostable ligase; Fidelity
First Page Preview
Quantification and improvement of error rate during ligase detection reaction
Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Bioscience and Bioengineering - Volume 109, Issue 2, February 2010, Pages 202–204
Authors
, , ,
Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering