fulltext.study @t Gmail

Biological detoxification of waste house wood hydrolysate using Ureibacillus thermosphaericus for bioethanol production

Paper ID Volume ID Publish Year Pages File Format Full-Text
21650 43233 2008 6 PDF Available
Title
Biological detoxification of waste house wood hydrolysate using Ureibacillus thermosphaericus for bioethanol production
Abstract

Hydrolysates of lignocelluloses hydrolyzed by diluted sulfuric acid contain toxic compounds that inhibit ethanol production by Saccharomyces cerevisiae and the ethanologenic recombinant Escherichia coli KO11. We investigated the biological detoxification of a hydrolysate of waste house wood (WHW) by a thermophilic bacterium, Ureibacillus thermosphaericus. When the hydrolysate was treated with this bacterium at 50°C for 24 h, the ethanol production rate by S. cerevisiae increased markedly and was comparable to that for the hydrolysate treated with an excess amount of calcium hydroxide (overliming). Chromatographic analysis of synthetic hydrolysates containing furfural or 5-hydroxymethyl furfural that are considered to be major toxic compounds in hydrolysates revealed that U. thermosphaericus degrades these compounds. In the WHW hydrolysates, however, the concentrations of these compounds were not decreased markedly by the bacterium. These results suggest that the bacterium degrades minor but more toxic compounds or phenolic compounds in the WHW hydrolysates. The combination of bacterial and overliming treatments of hydrolysates minimized significantly the decrease in ethanol production rate by E. coli KO11 as fermentation proceeded. Because the bacterium grows rapidly and does not consume sugars, our biological detoxification should be useful for bioethanol production from acid hydrolysates of lignocelluloses.

Keywords
bioethanol; waste house wood; detoxification; Ureibacillus thermosphaericus; Saccharomyces cerevisiae; Escherichia coli KO11
First Page Preview
Biological detoxification of waste house wood hydrolysate using Ureibacillus thermosphaericus for bioethanol production
Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Bioscience and Bioengineering - Volume 106, Issue 2, August 2008, Pages 128–133
Authors
, , , , ,
Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering