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Applicability of random cloning method to analyze microbial community in full-scale anaerobic digesters

Paper ID Volume ID Publish Year Pages File Format Full-Text
21651 43233 2008 7 PDF Available
Title
Applicability of random cloning method to analyze microbial community in full-scale anaerobic digesters
Abstract

Microbial communities were analyzed in six full-scale anaerobic digesters and a lab-scale digester using the random cloning method. The purposes were to confirm the applicability, reproducibility, and error range of this method; to discuss the difference in the dominant microbes determined by this method in different operational conditions of temperature (mesophilic and thermophilic) and substrate (garbage, sewage sludge, and livestock waste); and to determine key microbes in each digester. Each sample was analyzed in triplicate. In one of the samples, 373 clones were analyzed to study the composition of microbial community in the digester. Time course analysis was conducted from the start-up period for approximately one year in one of the digesters. Similar microbial diversity was obtained corresponding to the type of substrate change (sewage sludge to garbage). Operational taxonomic units (OTUs) closely related to Coprothermobacter sp. and unidentified bacterium clones TUG14 and TUG22 disappeared during the first 40 d, while OTUs closely related to Bacillus sp. and Clostridium sp. increased later. Microbial diversity in digesters is strongly affected by the operational conditions, and similar microbial diversity can be obtained in triplicate analysis and under similar operational conditions. The present study verified the applicability of this method to discuss the overall difference in microbial communities.

Keywords
16S rDNA; random cloning; microbial community; anaerobic digester; full-scale; lab-scale; temperature; substrate
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Applicability of random cloning method to analyze microbial community in full-scale anaerobic digesters
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Bioscience and Bioengineering - Volume 106, Issue 2, August 2008, Pages 134–140
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us