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Decrease in antithrombin III fucosylation by expressing GDP-fucose transporter siRNA in Chinese hamster ovary cells

Paper ID Volume ID Publish Year Pages File Format Full-Text
21656 43233 2008 6 PDF Available
Title
Decrease in antithrombin III fucosylation by expressing GDP-fucose transporter siRNA in Chinese hamster ovary cells
Abstract

The full-length cDNA of GDP-fucose transporter (GFT) was cloned from Chinese hamster ovary (CHO) 13Dā€“35D cells. The predicted product consisted of 366 amino acids with 86% and 92% similarities to human GFT and mouse GFT, respectively. When synthetic small interfering RNA (siRNA) against CHO GFT was introduced into human antithrombin III (AT-III)-producing recombinant CHO cells, defucosylated AT-III increased by 30ā€“40% after 3-d incubation. A GFT siRNA expression plasmid was also constructed to defucosylate recombinant AT-III. CHO cells, which were transfected with the siRNA expression plasmid, showed approximately 75% reduction of Golgi-GDP fucose, resulting in 10ā€“40% increase in defucosylated AT-III.

Keywords
Chinese hamster ovary cells; fucosylation; small interfering RNA; antithrombin III; GDP-fucose transporter
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Decrease in antithrombin III fucosylation by expressing GDP-fucose transporter siRNA in Chinese hamster ovary cells
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Bioscience and Bioengineering - Volume 106, Issue 2, August 2008, Pages 168ā€“173
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
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