fulltext.study @t Gmail

Kinetic modeling and sensitivity analysis of xylose metabolism in Lactococcus lactis IO-1

Paper ID Volume ID Publish Year Pages File Format Full-Text
21670 43234 2009 9 PDF Available
Title
Kinetic modeling and sensitivity analysis of xylose metabolism in Lactococcus lactis IO-1
Abstract

We proposed a kinetic simulation model of xylose metabolism in Lactococcus lactis IO-1 that describes the dynamic behavior of metabolites using the simulator WinBEST-KIT. This model was developed by comparing the experimental time-course data of metabolites in batch cultures grown in media with initial xylose concentrations of 20.3–57.8 g/l with corresponding calculated data. By introducing the terms of substrate activation, substrate inhibition, and product inhibition, the revised model showed a squared correlation coefficient (r2) of 0.929 between the experimental time-course of metabolites and the calculated data. Thus, the revised model is assumed to be one of the best candidates for kinetic simulation describing the dynamic behavior of metabolites. Sensitivity analysis revealed that pyruvate flux distribution is important for higher lactate production. To confirm the validity of our kinetic model, the results of the sensitivity analysis were compared with enzyme activities observed during increasing lactate production by adding natural rubber serum powder to the xylose medium. The experimental results on pyruvate flux distribution were consistent with the prediction by sensitivity analysis.

Keywords
Lactic acid fermentation; Xylose; Lactococcus lactis IO-1; WinBEST-KIT; Kinetic simulation model; Sensitivity analysis
First Page Preview
Kinetic modeling and sensitivity analysis of xylose metabolism in Lactococcus lactis IO-1
Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Bioscience and Bioengineering - Volume 108, Issue 5, November 2009, Pages 376–384
Authors
, , , , , , , ,
Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering