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Effects of siRNAs targeting the spacer sequence of plant RNAi vectors on the specificity and efficiency of RNAi

Paper ID Volume ID Publish Year Pages File Format Full-Text
21680 43234 2009 3 PDF Available
Title
Effects of siRNAs targeting the spacer sequence of plant RNAi vectors on the specificity and efficiency of RNAi
Abstract

Small interfering RNAs (siRNAs) generated from the spacer region of hairpin RNAs were not detected in the RNA interference (RNAi) plants targeting the fatty acid desaturase gene. The expression of the desaturase gene was stably suppressed even when siRNAs targeting the spacer sequences were introduced into this plant.

Keywords
α-Linolenic acid; Fatty acid desaturase; RNA interference; siRNA; Spacer
First Page Preview
Effects of siRNAs targeting the spacer sequence of plant RNAi vectors on the specificity and efficiency of RNAi
Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Bioscience and Bioengineering - Volume 108, Issue 5, November 2009, Pages 435–437
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering