fulltext.study @t Gmail

Novel reporter cell line to analyze cytokine-mediated expression regulation of c-myc gene

Paper ID Volume ID Publish Year Pages File Format Full-Text
21681 43234 2009 3 PDF Available
Title
Novel reporter cell line to analyze cytokine-mediated expression regulation of c-myc gene
Abstract

Growth-promoting cytokines induce expression of nuclear proto-oncogenes that play critical roles in the regulation of cell proliferation. c-myc gene is one of those nuclear proto-oncogenes, whose regulation mechanisms of cytokine-mediated expression are not fully understood. Here, I generated a green fluorescent protein (GFP) reporter system that faithfully reflects interleukin-3 (IL-3)-induced c-myc gene expression. Flowcytometric analysis revealed cytokine-specific expression of reporter GFP. Kinetics of GFP mRNA expression was similar to that of endogenous c-myc mRNA. The reporter cell line will be a useful tool for studies of cell proliferation regulation through analysis of cytokine-induced c-myc gene expression.

Keywords
c-myc; Cytokine; Epidermal growth factor; Green fluorescent protein; Interleukin-3; Reporter gene
First Page Preview
Novel reporter cell line to analyze cytokine-mediated expression regulation of c-myc gene
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us
Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Bioscience and Bioengineering - Volume 108, Issue 5, November 2009, Pages 438–440
Authors
,
Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us