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Insertional chromatin immunoprecipitation: A method for isolating specific genomic regions

Paper ID Volume ID Publish Year Pages File Format Full-Text
21683 43234 2009 4 PDF Available
Title
Insertional chromatin immunoprecipitation: A method for isolating specific genomic regions
Abstract

We established a novel method, insertional chromatin immunoprecipitation (iChIP), for isolation of specific genomic regions. In iChIP, specific genomic domains are immunoprecipitated with antibody against a tag, which is fused to the DNA-binding domain of an exogenous DNA-binding protein, whose recognition sequence is inserted into the genomic domains of interest. The iChIP method will be a useful tool for dissecting chromatin structure of genomic region of interest.

Keywords
Chromatin structure; Purification; iChIP; Epigenetics; Transcriptional regulation
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Bioscience and Bioengineering - Volume 108, Issue 5, November 2009, Pages 446–449
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us