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Expression and purification of the recombinant mustard trypsin inhibitor 2 (MTI2) in Escherichia coli

Paper ID Volume ID Publish Year Pages File Format Full-Text
21687 43235 2009 4 PDF Available
Title
Expression and purification of the recombinant mustard trypsin inhibitor 2 (MTI2) in Escherichia coli
Abstract

The mustard trypsin inhibitor 2, MTI2, was expressed in Escherichia coli. A specific procedure for its production and purification is described. The recombinant protein was recovered by protein extraction from the insoluble fraction, then renatured and purified by ion exchange and gel filtration chromatography. Finally, the inhibitory activity against trypsin was also determined.

Keywords
Heterologous expression; Serine proteinase inhibitor; Sinapis alba L.; Trypsin inhibition; Escherichia coli
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Expression and purification of the recombinant mustard trypsin inhibitor 2 (MTI2) in Escherichia coli
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Bioscience and Bioengineering - Volume 108, Issue 4, October 2009, Pages 282–285
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us