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Simple metabolite extraction method for metabolic profiling of the solid-state fermentation of Aspergillus oryzae

Paper ID Volume ID Publish Year Pages File Format Full-Text
21708 43236 2010 5 PDF Available
Title
Simple metabolite extraction method for metabolic profiling of the solid-state fermentation of Aspergillus oryzae
Abstract

Solid-state culture of microorganisms is an important style of culture both in the traditional food industry and in the modern fermentation industry. We propose here a simple method for metabolite extraction from the solid-state fermentation of a filamentous fungus, Aspergillus oryzae, which is known as rice-koji. To evaluate the efficiency of metabolite extraction, liquid chromatography–mass spectrometry (LC/MS) was used for simultaneous detection of a wide range of metabolites including amino acids, organic acids and vitamins, which are of interest in rice-koji making. Among eleven different metabolite extraction methods tested, we found that extraction using acetonitrile/water (50:50), coupled with boiling at 70 °C for 5 min, was advantageous in terms of both extraction yield and metabolism quenching. We consider that our method provides a technical basis for metabolome analysis of a solid-state fermentation.

Keywords
Aspergillus oryzae; Koji; Solid-state fermentation; Metabolite extraction; Liquid chromatography–mass spectrometry (LCMS)
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Simple metabolite extraction method for metabolic profiling of the solid-state fermentation of Aspergillus oryzae
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Bioscience and Bioengineering - Volume 110, Issue 6, December 2010, Pages 665–669
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us