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Contribution of ethanol-tolerant xylanase G2 from Aspergillus oryzae on Japanese sake brewing

Paper ID Volume ID Publish Year Pages File Format Full-Text
21711 43236 2010 5 PDF Available
Title
Contribution of ethanol-tolerant xylanase G2 from Aspergillus oryzae on Japanese sake brewing
Abstract

We purified three xylanase isozymes (XynF1, XynF3 and XynG2) from a solid-state Aspergillus oryzae RIB128 culture using chromatography. The results of our sake-brewing experiment, in which we used exogenously supplemented enzymes, revealed that only XynG2 improved the alcohol yield and the material utilization. The alcohol yield of the XynG2 batch displayed an increase of 4.4% in comparison to the control, and the amount of sake cake decreased by 4.6%. The contribution of XynG2 was further confirmed through our brewing experiment in which we used the yeast heterogeneously expressing fungal xylanase isozymes. Interestingly XynG1, an enzyme with a XynG2-like sequence that is more vulnerable to ethanol, did not improve the sake-mash fermentation. The stability of XynG2 in ethanol was prominent, and it retained most of its original activity after we exposed it to 80% ethanol for 30 min, whereas the stability of the other isozymes in ethanol, including XynG1, was much lower (20–25% ethanol). We concluded, therefore, that the improvement of material utilization achieved with XynG2 is primarily attributable to its characteristically high stability in ethanol, thereby, effectively degrading rice endosperm cell walls under high-alcohol conditions such as a sake-mash environment.

Keywords
Aspergillus oryzae; Xylanase; Ethanol stability; Alcohol yield; Material utilization; Sake-mash fermentation
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Contribution of ethanol-tolerant xylanase G2 from Aspergillus oryzae on Japanese sake brewing
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Bioscience and Bioengineering - Volume 110, Issue 6, December 2010, Pages 679–683
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us