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Glucose transporter mediation responsible for morphological changes of human epithelial cells on glucose-displayed surfaces

Paper ID Volume ID Publish Year Pages File Format Full-Text
21722 43237 2008 8 PDF Available
Title
Glucose transporter mediation responsible for morphological changes of human epithelial cells on glucose-displayed surfaces
Abstract

Cellular morphology is one of the important factors for coordinating cell signaling. In this study, the morphological variation via glucose transporter (GLUT)-mediated anchoring was investigated in the cultures of human mammary epithelial cells in the presence or absence of insulin on culture surfaces with the changed ratios of d- and l-glucose displayed. With increasing ratio of d-glucose displayed on the surfaces, the cells showed a stretched shape in the culture with 10 μg/cm3 insulin, reaching the highest extent of cell stretching at 100% d-glucose display, whereas round cells were predominant at 0% d-glucose display. In the absence of insulin, on the other hand, the extent of cell stretching showed a concave profile in terms of the ratio of d-glucose displayed, the extent being highest at 50% d-glucose display. Blocking of integrin α5β1 or GLUTs1 and 4 on the cells using corresponding antibodies revealed that the primary mechanism for cell attachment was based on integrin-mediated binding, and that GLUTs1 and 4 contributed largely to morphological changes of cells. Confocal microscopy further revealed that GLUT4 localization occurred in response to d-glucose display as well as insulin addition. In the absence of insulin, GLUT4 spots were extensively observed in the cell body regardless of whether d-glucose was displayed or not. However, in the presence of insulin, the broad distribution of GLUT4 appeared on the basal and apical sides of cells at 100% d-glucose display, in contrast with its localization only on the apical side of cells at 0% d-glucose display. These results suggest that the quantitative balance between GLUTs on the cytoplasmic membrane and d-glucose displayed on a culture surface determines the cell morphology, as explained by the receptor saturation model.

Keywords
human epithelial cells; glucose-displayed surface; glucose transporter-mediated anchoring; morphological change; cellular roundness; receptor saturation model
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Glucose transporter mediation responsible for morphological changes of human epithelial cells on glucose-displayed surfaces
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Bioscience and Bioengineering - Volume 105, Issue 4, April 2008, Pages 319–326
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us