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High-level expression of recombinant human nerve growth factor beta in milk of nontransgenic rabbits

Paper ID Volume ID Publish Year Pages File Format Full-Text
21723 43237 2008 8 PDF Available
Title
High-level expression of recombinant human nerve growth factor beta in milk of nontransgenic rabbits
Abstract

The technology for the large-scale production of therapeutic recombinant proteins remains a challenge in the biopharmaceutical industry. In this study, we reported a nontransgenic approach to producing a large quantity of human nerve growth factor beta (hNGF-β) in rabbit milk by employing a recombinant adenoviral expression system. After directly instilling hNGF-β recombinant adenoviruses into rabbit mammary glands, a polypeptide with a molecular weight of 13.2 kDa was detected in rabbit milk. The maximal expression level of hNGF-β reached 346 μg/ml. The biological activity of recombinant hNGF-β was confirmed using PC12 cells and cultures of dorsal root ganglion neurons from chicken embryos. Our data suggest that instilling recombinant adenovirus directly into the mammary gland of mammals is an efficient approach to producing a large quantity of hNGF-β.

Keywords
nerve growth factor; human nerve growth factor beta; adenovirus; recombinant protein; mammary gland; milk
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High-level expression of recombinant human nerve growth factor beta in milk of nontransgenic rabbits
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Bioscience and Bioengineering - Volume 105, Issue 4, April 2008, Pages 327–334
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us