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Purification and properties of family-10 endo-1,4-β-xylanase from Penicillium citrinum and structural organization of encoding gene

Paper ID Volume ID Publish Year Pages File Format Full-Text
21729 43237 2008 8 PDF Available
Title
Purification and properties of family-10 endo-1,4-β-xylanase from Penicillium citrinum and structural organization of encoding gene
Abstract

An extracellular endo-1,4-β-xylanase was purified from the culture filtrate of a filamentous fungus, Penicillium citrinum strain FERM P-15944, grown on birch-wood xylan. The purified enzyme showed a single band on SDS–PAGE with an apparent Mr of 31.6 kDa. The xylanase activity was optimal at pH 6.0 and 50°C. Southern blot analysis indicated that the xylanase gene (xynB) was present as a single copy in the genome. The genomic DNA and cDNAs encoding this protein were cloned and sequenced. An open reading frame of xynB was interrupted by nine introns and encoded a presumed prepropeptide of 25 amino acids and a mature protein of 302 amino acids. Sequence alignment and the constructed neighbor-joining tree showed that the P. citrinum enzyme belongs to glycoside hydrolase family 10 and is closely related to several other xylanases from penicillia and black aspergilli. The xynB cDNA was functionally expressed in the methylotrophic yeast Pichia pastoris.

Keywords
endo-1,4-β-xylanase; extracellular enzyme; glycoside hydrolase family 10; Penicillium citrinum; phylogenetic analysis; xylan
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Purification and properties of family-10 endo-1,4-β-xylanase from Penicillium citrinum and structural organization of encoding gene
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Bioscience and Bioengineering - Volume 105, Issue 4, April 2008, Pages 367–374
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us