fulltext.study @t Gmail

Production of (S)-2-chloropropionate by asymmetric reduction of 2-chloroacrylate with 2-haloacrylate reductase coupled with glucose dehydrogenase

Paper ID Volume ID Publish Year Pages File Format Full-Text
21740 43237 2008 3 PDF Available
Title
Production of (S)-2-chloropropionate by asymmetric reduction of 2-chloroacrylate with 2-haloacrylate reductase coupled with glucose dehydrogenase
Abstract

(S)-2-Chloropropionate is a synthetic intermediate for phenoxypropionic acid herbicides. We constructed a system for asymmetric reduction of 2-chloroacrylate to produce (S)-2-chloropropionate with recombinant Escherichia coli cells producing 2-haloacrylate reductase from Burkholderia sp. WS and an NADPH regeneration system. The system provided 37.4 g/l (S)-2-chloropropionate in more than 99.9% e.e.

Keywords
2-haloacrylate reductase; 2-chloroacrylate; S)-2-chloropropionate; phenoxypropionic acid herbicides; asymmetric reduction; chiral synthon
First Page Preview
Production of (S)-2-chloropropionate by asymmetric reduction of 2-chloroacrylate with 2-haloacrylate reductase coupled with glucose dehydrogenase
Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Bioscience and Bioengineering - Volume 105, Issue 4, April 2008, Pages 429–431
Authors
, , , , , ,
Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering