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Effects of non-B DNA sequences on transgene expression

Paper ID Volume ID Publish Year Pages File Format Full-Text
21745 43238 2009 4 PDF Available
Title
Effects of non-B DNA sequences on transgene expression
Abstract

DNA conformation may be an important factor affecting gene transcription. In this study, we examined how DNA sequences with unusual conformations affect transgene expression. A30 and (CG)15 sequences that can adopt the B′ and Z conformations, respectively, were introduced into a β-actin promoter. Luciferase plasmids containing the manipulated promoter were transfected into NIH3T3 cells by electroporation and were delivered into mouse livers with a hydrodynamics-based injection. Expression from plasmid with the (CG)15 sequence was multiple times higher than expression from control plasmid DNA. The A30 sequence also tended to enhance expression. These results suggest that non-B DNA sequences could improve transgene expression in cells.

Keywords
Non-B DNA; Transgene expression; Electroporation; Hydrodynamics-based injection; Plasmid DNA
First Page Preview
Effects of non-B DNA sequences on transgene expression
Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Bioscience and Bioengineering - Volume 108, Issue 1, July 2009, Pages 20–23
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering