fulltext.study @t Gmail

Cloning and expression of GH11 xylanase gene from Aspergillus fumigatus MKU1 in Pichia pastoris

Paper ID Volume ID Publish Year Pages File Format Full-Text
21746 43238 2009 6 PDF Available
Title
Cloning and expression of GH11 xylanase gene from Aspergillus fumigatus MKU1 in Pichia pastoris
Abstract

A xylanase gene, xynf11a of Aspergillus fumigatus MKU1 was cloned and expressed in Pichia pastoris X33. Two exons of the xynf11a gene were amplified separately and fused by overlap extension PCR. The fused product was cloned in yeast expression vector pPICZB and expressed in P. pastoris under the control of the AOX1 promoter. P. pastoris transformants expressing recombinant xylanases were selected on xylan agar plate and their ability to produce the xylanase was evaluated in flask cultures. P. pastoris X33 (pZBxynf11aFP) efficiently secreted the recombinant xylanase into the medium and produced the high level of xylanase activity (14 U/ml) after 96 h of growth. The recombinant xylanase produced by P. pastoris showed maximum activity at pH 6.0 and temperature 60 °C. The recombinant xylanase did not exhibit any cellulase activity and hence it could be potentially used for pretreatment of paper pulp before bleaching.

Keywords
Xylanase; Aspergillus fumigatus; Overlap extension PCR; Expression; Pichia pastoris
First Page Preview
Cloning and expression of GH11 xylanase gene from Aspergillus fumigatus MKU1 in Pichia pastoris
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us
Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Bioscience and Bioengineering - Volume 108, Issue 1, July 2009, Pages 24–29
Authors
, , , ,
Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us