fulltext.study @t Gmail

Analysis of genes encoding the 2,4-dichlorophenoxyacetic acid-degrading enzyme from Sphingomonas agrestis 58-1

Paper ID Volume ID Publish Year Pages File Format Full-Text
21752 43238 2009 4 PDF Available
Title
Analysis of genes encoding the 2,4-dichlorophenoxyacetic acid-degrading enzyme from Sphingomonas agrestis 58-1
Abstract

A 2,4-dichlorophenoxy acetic acid (2,4-D)-degrading bacterium, strain 58-1, was newly isolated from soil samples collected in the Fukuoka Prefecture, Japan, and grown on an enrichment culture medium containing 2,4-D as the sole carbon source. Phylogenic analysis identified strain 58-1 as Sphingomonas agrestis. In 2,4-D degraders, classes I, II, and III inherit the tfdA, cadA, and tfdAα genes, respectively, and the results from degenerate-PCR indicated that this strain belongs to the class II degraders. A clone that includes the cadA gene homolog of S. agrestis 58-1 was screened from a library by using the PCR amplified fragment as a DNA probe. The cloned fragment was sequenced and found to consist of 5043 nucleotides and include 3 open reading frames (orfs). The orf1, orf2, and orf3 genes encode polypeptides consisting of 412, 448, and 177 amino acids, respectively. The Orf2 product shares a high degree of sequence similarity (92%) with the large subunit of 2,4-D oxygenase from the Bradyrhizobium sp. strain HW13, which belongs to the class III 2,4-D degraders, while the orf3 product shared 63% sequence similarity with the small subunit of 2,4-D oxygenase from the strain HW13. The results of the functional expression analysis using various deletion mutants in Escherichia coli revealed that the expression of both orf2 and orf3 genes, but not orf1, is essential for the conversion of 2,4-D to 2,4-DCP. From these results, we conclude the first isolation of 2,4-D oxygenase genes from a class II 2,4-D degrader.

Keywords
2,4-dichlorophenoxyacetic acid-degrading bacterium; 2,4-D oxygenase; cadAB genes; Sphingomonas agrestis 58-1; Functional expression; Deletion mutants
First Page Preview
Analysis of genes encoding the 2,4-dichlorophenoxyacetic acid-degrading enzyme from Sphingomonas agrestis 58-1
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us
Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Bioscience and Bioengineering - Volume 108, Issue 1, July 2009, Pages 56–59
Authors
, , ,
Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us