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Fermentative production of (R)-(−)-3-hydroxybutyrate using 3-hydroxybutyrate dehydrogenase null mutant of Ralstonia eutropha and recombinant Escherichia coli

Paper ID Volume ID Publish Year Pages File Format Full-Text
21880 43241 2006 6 PDF Available
Title
Fermentative production of (R)-(−)-3-hydroxybutyrate using 3-hydroxybutyrate dehydrogenase null mutant of Ralstonia eutropha and recombinant Escherichia coli
Abstract

Two systems, one using an (R)-(−)-3-hydroxybutyrate dehydrogenase (BDH) null mutant of Ralstonia eutropha and the other using a recombinant Escherichia coli strain containing a synthetic poly[(R)-(−)-3-hydroxybutyrate] (PHB) operon and an extracellular PHB depolymerase gene, were used for the fermentative production of (R)-(−)-3-hydroxybutyrate (3HB). The concentration of 3HB in the culture supernatant of the mutant R. eutropha system reached about 30 mM after 5 d under anaerobic conditions, although it was about 4-10 mM under aerobic conditions. On the other hand, the 3HB concentration in the culture supernatant of the recombinant E. coli system reached about 70 mM after 4 d, indicating that about 70% of the glucose added was converted to 3HB.

Keywords
(R)-(−)-3-hydroxybutyrate (3HB); poly[(R)-(−)-3-hydroxybutyrate] (PHB); Ralstonia eutropha; poly[(R)-(−)-3-hydroxybutyrate] (PHB) synthetic operon; poly[(R)-(−)-3-hydroxybutyrate] (PHB) depolymerase; Escherichia coli
First Page Preview
Fermentative production of (R)-(−)-3-hydroxybutyrate using 3-hydroxybutyrate dehydrogenase null mutant of Ralstonia eutropha and recombinant Escherichia coli
Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Bioscience and Bioengineering - Volume 102, Issue 6, December 2006, Pages 529–534
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering