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Characterization of polystyrene-binding peptides (PS-tags) for site-specific immobilization of proteins

Paper ID Volume ID Publish Year Pages File Format Full-Text
21900 43242 2010 5 PDF Available
Title
Characterization of polystyrene-binding peptides (PS-tags) for site-specific immobilization of proteins
Abstract

In this study, we characterized polystyrene-binding peptides (PS-tags) that possess a specific binding affinity for hydrophilic polystyrene (phi-PS) plates. Both the FITC-labeled PS19-1 (RAFIASRRIKRP) and PS19-6 (RIIIRRIRR) peptides showed strong binding affinity for commercially available hydrophilic, but not hydrophobic, PS plates in the presence of the non-ionic surfactant Tween 20. The dissociation constants (Kd) of the PS19-1 and PS19-6 peptides for the hydrophilic PS-A plate were 169 and 86 nM, respectively, and the Kd of both peptides increased with the concentration of NaCl or urea. Based on adsorption yield and residual activity of glutathione S-transferase (GST) after fusion with the PS19-6 peptide or its variants, it was found that the basic amino acid in the PS-tags, i.e., Arg was essential for the strong binding affinity of PS-tags in both the peptide and peptide-fused protein forms The aliphatic amino acids in PS19-6 and PS19-6L, such as Ile or Leu, were also effective. Thus, a series of PS-tags that possess this unusual feature, especially the peptides PS19-6 (RIIIRRIRR) and PS19-6L (RLLLRRLRR), are potential candidate affinity peptide tags for site-specific immobilization of proteins onto hydrophilic PS plates, which show potential as solid supports for protein-based biochips.

Keywords
Affinity peptide; PS-tag; Glutathione S-transferase (GST); Site-specific protein immobilization; Polystyrene
First Page Preview
Characterization of polystyrene-binding peptides (PS-tags) for site-specific immobilization of proteins
Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Bioscience and Bioengineering - Volume 109, Issue 6, June 2010, Pages 583–587
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering