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Refolding of Fully Reduced Bovine Pancreatic Trypsin

Paper ID Volume ID Publish Year Pages File Format Full-Text
21914 43243 2008 5 PDF Available
Title
Refolding of Fully Reduced Bovine Pancreatic Trypsin
Abstract

Refolding of bovine pancreatic trypsin was carried out. When starting with denatured S-S intact trypsin, the recovered activity attained was 95–100%. In contrast, the recovered activity after refolding denatured S-S reduced trypsin was considerably low compared with other proteases that have been worked with previously. Such low recovered activity was attributed to the small amount of fully reduced trypsin used as starting material for complete refolding. Taking this into account, a recovered activity of 86% could be achieved when using inhibitor-immobilized gels.

Keywords
protease refolding; bovine pancreatic trypsin; fully reduced trypsin; immobilized inhibitor
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Bioscience and Bioengineering - Volume 106, Issue 4, October 2008, Pages 345–349
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us