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A Comparison of Various Methods to Predict Bacterial Predilection for Organic Solvents Used as Reaction Media

Paper ID Volume ID Publish Year Pages File Format Full-Text
21916 43243 2008 6 PDF Available
Title
A Comparison of Various Methods to Predict Bacterial Predilection for Organic Solvents Used as Reaction Media
Abstract

Bacterial predilection for organic solvents is important in whole-cell biocatalysis in organic media. Although various methods of measuring bacterial hydrophobicity have been proposed, it is not fully determined whether they are applicable to the assessment of bacterial predilection for organic solvents in whole-cell biocatalytic processes. In this study, bacterial predilection for organic solvents was assessed by bacterial adhesion to hydrocarbon (BATH), contact angle measurement (CAM), hydrophobic interaction chromatography (HIC), and glass adhesion test (GAT). These methods were applied to the cultures of four bacterial species of industrial importance, namely, Rhodococcus opacus B-4, R. erythropolis PR4, Pseudomonas putida T-57, and Escherichia coli JM109, in organic media. Experimental results revealed that CAM assays could be used to predict the dispersibility of bacterial cells in anhydrous organic solvents. However, when bacteria were suspended in aqueous-organic (A/O) two-phase media, the results of BATH assays provided the most reliable assessment of bacterial predilection for organic solvents. This discrepancy noted between CAM and BATH assays was attributed to the effect of electrostatic interaction between bacteria and oil droplets. In A/O two-phase media, the accessibility of a water-immiscible dye, nile red, to the bacterial cell surface, correlated well with BATH assay results.

Keywords
cell-surface hydrophobicity; zeta potential; Rhodococcus opacus; organic-solvent-tolerant bacteria; water-immiscible chemicals
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A Comparison of Various Methods to Predict Bacterial Predilection for Organic Solvents Used as Reaction Media
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Bioscience and Bioengineering - Volume 106, Issue 4, October 2008, Pages 357–362
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us