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Molecular Cloning, Characterization, and Expression of an ω-3 Fatty Acid Desaturase Gene from Sapium sebiferum

Paper ID Volume ID Publish Year Pages File Format Full-Text
21919 43243 2008 6 PDF Available
Title
Molecular Cloning, Characterization, and Expression of an ω-3 Fatty Acid Desaturase Gene from Sapium sebiferum
Abstract

A full-length cDNA (SsFAD3) for an ω-3 fatty acid desaturase (ω-3 FAD) was cloned from Sapium sebiferum (L.) Roxb. using rapid amplification of cDNA ends and reverse transcription polymerase chain reaction methods. SsFAD3 contained a 1119-bp open reading frame encoding a 372-amino acid polypeptide. The genomic sequence region of the SsFAD3 ORF was composed of 8 exons and 7 introns, similar to other ω-3 FADs found in most plants. The amino acid sequence showed a higher identity with microsomal ω-3 FADs than plastidial ω-3 FADs. Southern blot analysis of SsFAD3 suggested the existence of a small gene family composed of several copies or closely linked genes. SsFAD3 transcripts were detected in shoots, roots, leaves, stems, and seeds, but were most abundant in shoots. The function of SsFAD3 was confirmed by the accumulation of α-linolenic acid (α-18:3) in Saccharomyces cerevisiae transformants.

Keywords
cloning; ω-3 fatty acid desaturase gene; Saccharomyces cerevisiae; Sapium sebiferum (L.) Roxb
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Molecular Cloning, Characterization, and Expression of an ω-3 Fatty Acid Desaturase Gene from Sapium sebiferum
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Bioscience and Bioengineering - Volume 106, Issue 4, October 2008, Pages 375–380
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us