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Cloning and Expression of Soluble Cytochrome c and Its Role in Polyvinyl Alcohol Degradation by Polyvinyl Alcohol-Utilizing Sphingopyxis sp. Strain 113P3

Paper ID Volume ID Publish Year Pages File Format Full-Text
21939 43244 2008 5 PDF Available
Title
Cloning and Expression of Soluble Cytochrome c and Its Role in Polyvinyl Alcohol Degradation by Polyvinyl Alcohol-Utilizing Sphingopyxis sp. Strain 113P3
Abstract

The gene encoding cytocrome c in the pva operon of Sphingopyxis sp. strain 113P3 was cloned, on the basis of the sequence of the gene for cytochrome c (GenBank accession no. AB190288). The deduced amino acid sequence of the gene showed homologies (37% and 47% identities) with two cytochromes c of different origins. The recombinant cytochrome c tagged with hexahistidines was expressed in the periplasm of Escherichia coli BL21(DE3) harboring pT-GroE, which was in accordance with the localization of cytochrome c in strain 113P3; the protein was purified to homogeneity. The purified recombinant cytochrome c was a monomeric protein with a molecular weight of 16.5 kDa. The oxidized and reduced forms of the protein showed absorption maxima at 409 nm and at 414, 520 and 550 nm, respectively. The recombinant cytochrome c was fully reduced by polyvinyl alcohol (PVA), coupled with a catalytic amount (1/10 molar concentration) of the recombinant PVA dehydrogenase (PVADH) of the same origin, suggesting that the cytochrome c involved in the pva operon is a physiological primary electron acceptor for PVADH and that PVA dehydrogenation is linked with the respiratory chain in Sphingopyxis sp. strain 113P3.

Keywords
Sphingopyxis sp. strain 113P3; cytochrome c; poly(vinyl alcohol) dehydrogenase; respiratory chain; periplasmic degradation
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Cloning and Expression of Soluble Cytochrome c and Its Role in Polyvinyl Alcohol Degradation by Polyvinyl Alcohol-Utilizing Sphingopyxis sp. Strain 113P3
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Bioscience and Bioengineering - Volume 105, Issue 2, February 2008, Pages 147–151
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us