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Purification and characterization of a thermostable xylanase from the brown-rot fungus Laetiporus sulphureus

Paper ID Volume ID Publish Year Pages File Format Full-Text
21951 43245 2009 5 PDF Available
Title
Purification and characterization of a thermostable xylanase from the brown-rot fungus Laetiporus sulphureus
Abstract

A thermostable extracellular xylanase was purified and characterized from brown-rot basidiomycete Laetiporus sulphureus, cultivated on biologically pretreated Pinus densiflora biomass. After three consecutive purification steps using DEAE, Mono Q, and Superdex 75 columns, the xylanase specific activity was found to be 72.4 U/mg, nine fold higher than that of the crude culture solution, purity was 96%, and the molecular mass determined to be 69.3 kDa. The optimal pH and temperature for xylanase activity were 3.0 and 80 °C, respectively. Although activity of xylanase was highest at 80 °C, it showed highest thermostability at 60 °C, retaining approximately 97% of its relative activity following incubation for 4 h. In the presence of 5 mM solution of CaCl2, the relative xylanase activity increased by 35.9%; however, it decreased significantly in the presence of 10 mM solution of Cu2+. Among the xylan-based substrates tested, purified L. sulphureus xylanase showed the highest activity on beechwood xylan. Thin-layer chromatography (TLC) experiments revealed that purified L. sulphureus xylanase is an endoxylanase that hydrolyzes xylotriose, xylotetraose, and xylopentaose but not xylobiose.

Keywords
Brown-rot basidiomycetes; Laetiporus sulphureus; Thermostable endoxylanase; Xylam; Pinus densiflora
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Purification and characterization of a thermostable xylanase from the brown-rot fungus Laetiporus sulphureus
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Bioscience and Bioengineering - Volume 107, Issue 1, January 2009, Pages 33–37
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us