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A dual-functional fibrous scaffold enhances P450 activity of cultured primary rat hepatocytes

Paper ID Volume ID Publish Year Pages File Format Full-Text
2196 103 2007 8 PDF Available
Title
A dual-functional fibrous scaffold enhances P450 activity of cultured primary rat hepatocytes
Abstract

We have designed a novel dual-functional electrospun fibrous scaffold comprising two fiber mesh layers that were modified differently to induce two separate biological responses from hepatocytes. The first fiber layer was galactosylated on the surface to mediate hepatocyte attachment, while the second layer was loaded with 3-methylcholanthrene (3-Mc) to enhance cytochrome P450 activity of hepatocytes. Primary rat hepatocytes cultured on the galactosylated fibrous scaffolds loaded with different concentrations of 3-Mc were compared for their cell attachment efficiency, albumin secretion activity and cytochrome P450-dependent 7-ethoxycoumarin O-deethylase activity. This hybrid fibrous scaffold mediated hepatocyte attachment with slightly lower efficiency (76 ± 2.3%) than a single-layer galactosylated fibrous scaffold (84 ± 3.5%). More importantly, the cytochrome P450 activity of the hepatocytes cultured on the hybrid scaffold correlated well with the 3-Mc loading level. The results also showed that transfer of 3-Mc to hepatocytes through direct cell–fiber contact was the dominant transport route, with the induced cytochrome P450 activity being 1.9- to 4.8-fold higher than that of transfer of 3-Mc to hepatocytes via dissolution from fibers to medium. This study demonstrates the feasibility of creating multi-functional fibrous scaffolds that serve both as an adhesive substrate and as a delivery vehicle for bioactive molecules.

Keywords
Electrospun fiber; Surface modification; Drug encapsulation; Hepatocyte culture
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A dual-functional fibrous scaffold enhances P450 activity of cultured primary rat hepatocytes
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Publisher
Database: Elsevier - ScienceDirect
Journal: Acta Biomaterialia - Volume 3, Issue 5, September 2007, Pages 643–650
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us