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Higher-level accumulation of foreign gene products in transgenic rice seeds by the callus-specific selection system

Paper ID Volume ID Publish Year Pages File Format Full-Text
21960 43245 2009 6 PDF Available
Title
Higher-level accumulation of foreign gene products in transgenic rice seeds by the callus-specific selection system
Abstract

Transgenic rice plants that accumulate the hypocholesterolemic pentapeptide lactostatin (IIAEK) as a fusion protein with the endosperm seed storage protein glutelin were developed. Plants were selected from Agrobacterium-mediated transformants using a mutated-rice acetolactate synthase (mALS) marker protein expressed under the control of the rice callus-specific promoter (CSP) (CSP:mALS). Lactostatin accumulation levels were compared in mature seeds of 78 independent transgenic rice lines selected for either the CSP:mALS gene cassette or hygromycin phosphotransferase (HPT) under the control of the CaMV 35S promoter (35S:HPT). Transgenic rice seeds harboring a CSP:mALS gene cassette accumulated more (approximately 120–200% on average) lactostatin than those with a 35S:HPT gene cassette, and accumulation was largely independent of transgene copy number. Furthermore, transgenic rice seeds that were selected by HPT under the control of CSP (CSP:HPT) also accumulated more lactostatin (approximately 160% on average) than transgenic rice seeds with the 35S:HPT gene cassette. These results indicate that high-value bioactive peptides such as lactostatin can be produced at higher levels using callus-specific selection than by conventional constitutive selection. Specific expression of a marker gene at the selection stage may thus result in increased target peptide accumulation levels in seeds.

Keywords
Agrobacterium; Callus-specific promoter; Endosperm; Oriza sativa L.; Selectable marker; 35S promoter; Transgenic rice
First Page Preview
Higher-level accumulation of foreign gene products in transgenic rice seeds by the callus-specific selection system
Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Bioscience and Bioengineering - Volume 107, Issue 1, January 2009, Pages 78–83
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering