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Mutagenesis of longer inserts by the ligation of two PCR fragments amplified with a mutation primer

Paper ID Volume ID Publish Year Pages File Format Full-Text
21963 43245 2009 3 PDF Available
Title
Mutagenesis of longer inserts by the ligation of two PCR fragments amplified with a mutation primer
Abstract

We report a method for efficient mutagenesis of DNA in large vectors without subcloning. Two segments of the target DNA sequence, one having a mutation introduced via a mutant primer, were amplified by PCR and then the purified fragments were ligated to a vector. The mutation efficiency was nearly 100%.

Keywords
Polymerase chain reaction (PCR); Mutagenesis; Yeast; Expression vector; Deletion; Insertion; Substitution
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Mutagenesis of longer inserts by the ligation of two PCR fragments amplified with a mutation primer
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Bioscience and Bioengineering - Volume 107, Issue 1, January 2009, Pages 95–97
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us