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Analysis of hemin effect on lactate reduction in Lactococcus lactis

Paper ID Volume ID Publish Year Pages File Format Full-Text
21970 43246 2007 6 PDF Available
Title
Analysis of hemin effect on lactate reduction in Lactococcus lactis
Abstract

Lactococcus lactis is a facultative anaerobic microorganism that produces lactate as the major product, and acetate and acetoin as by-products; some strains of this species produce an antimicrobial compound, nisin. Lactate has a strong inhibitory effect on L. lactis growth. On the other hand, hemin has a suppressive effect on lactate production during L. lactis growth under aerobic condition. To achieve the optimum effect of hemin on lactate amount reduction in L. lactis ATCC11454, cultures entailing various conditions were performed with and without hemin. In the culture with hemin, L. lactis growth and lactate reduction improved compared with those in the culture without hemin; that is, lactate production was suppressed by 1.8- and 1.3-fold under batch and fed-batch cultures, respectively. In microaerobic fed-batch culture with hemin, lactate production was sufficiently suppressed. This result suggests that microaerobic fed-batch culture could be applied to the maintenance of the low lactate amount. Under this condition, metabolic shift was observed from lactate to acetoin and acetate. However, no increase in nisin production was observed even though lactate production could significantly decrease in L. lactis ATCC11454.

Keywords
Lactococcus lactis; hemin; lactate; microaerobic condition; nisin; metabolic shift
First Page Preview
Analysis of hemin effect on lactate reduction in Lactococcus lactis
Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Bioscience and Bioengineering - Volume 103, Issue 6, June 2007, Pages 529–534
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering