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Characterization of efficient aerobic denitrifiers isolated from two different sequencing batch reactors by 16S-rRNA analysis

Paper ID Volume ID Publish Year Pages File Format Full-Text
21975 43246 2007 5 PDF Available
Title
Characterization of efficient aerobic denitrifiers isolated from two different sequencing batch reactors by 16S-rRNA analysis
Abstract

By adopting two sequencing batch reactors (SBRs) A and B, nitrate as the substrate, and the intermittent aeration mode, activated sludge was domesticated to enrich aerobic denitrifiers. The pHs of reactor A were approximately 6.3 at DOs 2.2–6.1 mg/l for a carbon source of 720 mg/l COD; the pHs of reactor B were 6.8–7.8 at DOs 2.2–3.0 mg/l for a carbon source of 1500 mg/l COD. Both reactors maintained an influent nitrate concentration of 80 mg/l NO3−-N. When the total inorganic nitrogen (TIN) removal efficiency of both reactors reached 60%, aerobic denitrifier accumulation was regarded completed. By bromthymol blue (BTB) medium, 20 bacteria were isolated from the two SBRs and DNA samples of 8 of these 20 strains were amplified by PCR and processed for 16SrRNA sequencing. The obtained results were analysed by a Blast similarity search of the GenBank database, and constructing a phylogenetic tree for identification by comparison. The 8 bacteria were found to belong to the genera Pseudomonas, Delftia, Herbaspirillum and Comamonas. At present, no Delftia has been reported to be an aerobic denitrifier.

Keywords
aerobic denitrifiers; 16S rRNA; phylogenetic analysis; Delftia
First Page Preview
Characterization of efficient aerobic denitrifiers isolated from two different sequencing batch reactors by 16S-rRNA analysis
Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Bioscience and Bioengineering - Volume 103, Issue 6, June 2007, Pages 563–567
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering