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Biological properties of loop-replaced mutants of Bacillus thuringiensis mosquitocidal Cry4Aa

Paper ID Volume ID Publish Year Pages File Format Full-Text
21981 43247 2009 5 PDF Available
Title
Biological properties of loop-replaced mutants of Bacillus thuringiensis mosquitocidal Cry4Aa
Abstract

Cry4Aa produced by Bacillus thuringiensis subsp. israelensis (Bti) exhibits a specific toxicity to Anopheles, Aedes, and Culex larvae, which are vectors of serious diseases, and formulations of Bti are used worldwide for mosquito control. In general, domain II of the Cry toxin is believed to be important for target specificity, and three loops (loops 1, 2, and 3) in domain II have been studied extensively. In this report, to analyze the biological functions of loops 1, 2, and 3 of Cry4Aa, mutants were constructed in which one of the loops was replaced with either of the other two loops. A bioassay using Culex pipiens larvae revealed that the mosquitocidal activity was virtually lost upon replacement of loop2. The mutants in which loops 1 and/or 3 were replaced also showed decreased activity, but they still maintained some activities. This suggested that loop2, but not loops 1 and 3, was essential for the mosquitocidal activity of Cry4Aa. Proteolytic digestion revealed the involvement of loops in the stability of the Cry4Aa structure. No significant differences were observed in the amount of wild-type and mutant Cry4Aa bound to the BBMVs prepared from the C. pipiens larvae.

Keywords
Bacillus thuringiensis; Cry4Aa; Loop replaced mutants; Mosquitocidal toxicity; Culex pipiens; Genetic engineering
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Biological properties of loop-replaced mutants of Bacillus thuringiensis mosquitocidal Cry4Aa
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Bioscience and Bioengineering - Volume 108, Issue 3, September 2009, Pages 179–183
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us