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The attachment of catalase and poly-l-lysine to plasma immersion ion implantation-treated polyethylene

Paper ID Volume ID Publish Year Pages File Format Full-Text
2202 103 2007 10 PDF Available
Title
The attachment of catalase and poly-l-lysine to plasma immersion ion implantation-treated polyethylene
Abstract

Plasma immersion ion implantation (PIII) treatment of polyethylene increased the functional attachment of catalase and increased the retention of enzyme activity in comparison to untreated controls. The attached protein was not removed by SDS or NaOH, while that on the untreated surfaces was easily removed. Poly-l-lysine was found to attach in a similar way to the treated surface and could not be removed by NaOH, while it did not attach to the untreated surface. This indicates that a new binding mechanism, covalent in nature, is introduced by the plasma treatment. Surfaces treated with PIII maintained the catalase activity more effectively than surfaces plasma treated without PIII. The PIII-treated surface was hydrophilic compared to the untreated surface and retained its hydrophilic character better than surfaces subjected to a conventional plasma treatment process. The strong modification of a deeper region of the polymer than for conventional plasma treatments is believed to be responsible for both the enhanced hydrophilic character and for the increase in functional lifetime of the attached protein. The results show that PIII treatment of polymers increases their usefulness for protein microarrays.

Keywords
Protein adsorption; Catalase; Plasma immersion ion implantation; Polyethylene; Surface modification
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The attachment of catalase and poly-l-lysine to plasma immersion ion implantation-treated polyethylene
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Publisher
Database: Elsevier - ScienceDirect
Journal: Acta Biomaterialia - Volume 3, Issue 5, September 2007, Pages 695–704
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
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Price was $35.95
You save - $31
Price after discount Only $4.95
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Full-text PDF Download
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