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Stepwise Esterification of Phytosterols with Conjugated Linoleic Acid Catalyzed by Candida rugosa Lipase in Solvent-free Medium

Paper ID Volume ID Publish Year Pages File Format Full-Text
22041 43250 2008 4 PDF Available
Title
Stepwise Esterification of Phytosterols with Conjugated Linoleic Acid Catalyzed by Candida rugosa Lipase in Solvent-free Medium
Abstract

We conducted a near quantitative esterification of phytosterols from soybean oil deodorizer distillate with conjugated linoleic acid. We used a 1:1 molar ratio of sterols to conjugated linoleic acid. For that matter, stepwise addition of sterols was investigated. Total sterols were divided into several portions and added sequentially to the reaction mixture. Using this methodology, purities of up to 80% steryl esters were obtained that consumed more than 90% of the total conjugated linoleic acid. In addition, the effects of temperature, amount, and stability of lipase were also evaluated.

Keywords
Candida rugosa; conjugated linoleic acid; esterification; lipase; sterols
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Stepwise Esterification of Phytosterols with Conjugated Linoleic Acid Catalyzed by Candida rugosa Lipase in Solvent-free Medium
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Bioscience and Bioengineering - Volume 106, Issue 6, December 2008, Pages 559–562
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us