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Construction and Characterization of Single-Gene Chromosomes in Saccharomyces cerevisiae

Paper ID Volume ID Publish Year Pages File Format Full-Text
22042 43250 2008 5 PDF Available
Title
Construction and Characterization of Single-Gene Chromosomes in Saccharomyces cerevisiae
Abstract

Based on a previously developed PCR-mediated chromosome splitting method, a genome engineering technique was developed in haploid Saccharomyces cerevisiae for incorporating any desired chromosomal region into a chromosome that carries a single gene. Based on the viability of cells carrying an essential gene in such a construct, close physical proximity of two telomeres and a centromere does not appear to compromise gene function. Spontaneous loss of constructed single-gene chromosomes during vegetative growth was high (0.2–0.4 per cell division), suggesting the possibility of creating novel cells carrying single-gene chromosomes derived from various chromosomal regions in a variety of combinations by exploiting combinatorial loss.

Keywords
budding yeast; Cre-loxP system; chromosome instability; chromosome loss; chromosome splitting; mini-chromosome
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Construction and Characterization of Single-Gene Chromosomes in Saccharomyces cerevisiae
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Bioscience and Bioengineering - Volume 106, Issue 6, December 2008, Pages 563–567
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us