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Breeding of Industrial Diploid Yeast Strain with Chromosomal Integration of Multiple β-Glucosidase Genes

Paper ID Volume ID Publish Year Pages File Format Full-Text
22047 43250 2008 4 PDF Available
Title
Breeding of Industrial Diploid Yeast Strain with Chromosomal Integration of Multiple β-Glucosidase Genes
Abstract

We constructed a double auxotrophic OC-2 industrial diploid strain of Saccharomyces cerevisiae and introduced 4 copies of cell surface displaying β-glucosidase (BGL) genes into the chromosome. The engineered OC-2 strain showed 5-fold higher BGL activity compared with the yeast carrying 2 copies of BGL gene and directly produced ethanol from cellobiose.

Keywords
yeast; cellulosic ethanol; β-glucosidase; chromosomal integration; fermentation
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Breeding of Industrial Diploid Yeast Strain with Chromosomal Integration of Multiple β-Glucosidase Genes
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Bioscience and Bioengineering - Volume 106, Issue 6, December 2008, Pages 594–597
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us