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Synthetic matrix containing glucocorticoid and growth factor for chondrogenic differentiation of stem cells

Paper ID Volume ID Publish Year Pages File Format Full-Text
22066 43251 2009 6 PDF Available
Title
Synthetic matrix containing glucocorticoid and growth factor for chondrogenic differentiation of stem cells
Abstract

With the present study the aim was to assess the efficacy of synthetic matrix as specific drug carriers carrying encapsulation of cells for the differentiation using mesenchymal stem cells (MSCs). The poly(N-isopropylacrylamide-co-vinylimidazole); p(NiPAAm-co-VI)) matrix itself, synthetic matrix, without drugs was used as the control in order to determine the effects of these materials on differentiation. MSCs obtained from rabbit bone marrow were tested as a potential cell source for tissue regeneration encapsulated in synthetic matrix, in the presence or absence of the growth factor (TGF-β1) and dexamethasone (Dex). The encapsulated with cells and synthetic matrix were transplanted into a nude mouse as an injected form and cultured for up to 8 weeks. The specific gene expression was determined by RT-PCR analysis. The amount of cartilage-associated ECM proteins was examined by Safranin-O staining. These data indicate the potential use of TGF-β1 and Dex for reconstruction of neocartilage formation. We have concluded that synthetic matrix including TGF-β 1 and Dex has useful carrier as containing inducers of chondrogenic differentiation materials in the regeneration of tissue-engineered cartilage in vivo.

Keywords
Thermo-sensitive hydrogel; Dexamethasone; Transforming growth factor-β1 (TGF-β1); Chondrogenic differentiation; Mesenchymal stem cells
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Synthetic matrix containing glucocorticoid and growth factor for chondrogenic differentiation of stem cells
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Bioscience and Bioengineering - Volume 108, Issue 2, August 2009, Pages 168–173
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us